Human MYO9B ELISA Kit
Sensitivity
0.19 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00849
Synonyms
CELIAC4, MYO9B variant protein, myosin IXB, MYR5, Unconventional myosin-9b
Validation Data Gallery
Product Information
KE00849 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MYO9B ELISA kit is to be used to detect and quantify protein levels of endogenous human MYO9B. The assay recognizes human MYO9B. An antibody specific for human MYO9B has been pre-coated onto the microwells. The human MYO9B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MYO9B is added to detect the captured human MYO9B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MYO9B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.19 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4650 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 83%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 24.10 | 0.83 | 3.43 |
| 2 | 8 | 6.25 | 0.22 | 3.50 |
| 3 | 8 | 3.27 | 0.12 | 3.74 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.05 | 1.52 | 6.06 |
| 2 | 16 | 6.41 | 0.33 | 5.13 |
| 3 | 16 | 3.26 | 0.15 | 4.66 |
Background Information
MYO9B (Myosin IXB) is a member of the myosin superfamily of motor proteins that plays a crucial role in intracellular trafficking, cell migration, and cytoskeletal organization. It acts as a Rho GTPase-activating protein (RhoGAP), meaning it regulates the activity of RhoA, a small GTPase involved in cell signaling. This protein is highly expressed in immune cells, epithelial tissues, and the nervous system, where it contributes to processes such as phagocytosis, cell polarization, and barrier integrity. MYO9B is strongly associated with IBD, including Crohn's disease and ulcerative colitis, suggesting its involvement in the development of these conditions. Additionally, MYO9B's interaction with the actin cytoskeleton makes it essential for maintaining cellular structure and facilitating mechanotransduction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CELIAC4, MYO9B variant protein, myosin IXB, MYR5, Unconventional myosin-9b |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
