Human IMPDH1 ELISA Kit
Sensitivity
0.20 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00935 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IMPDH1 ELISA kit is to be used to detect and quantify protein levels of endogenous human IMPDH1. The assay recognizes human IMPDH1. An antibody specific for human IMPDH1 has been pre-coated onto the microwells. The human IMPDH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IMPDH1 is added to detect the captured human IMPDH1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IMPDH1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3614 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 86% | 80%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.52 | 0.92 | 4.27 |
| 2 | 8 | 5.21 | 0.09 | 1.74 |
| 3 | 8 | 2.68 | 0.08 | 3.07 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 20.77 | 1.30 | 6.24 |
| 2 | 16 | 5.21 | 0.09 | 1.68 |
| 3 | 16 | 2.64 | 0.09 | 3.58 |
Background Information
IMPDH1 (inosine monophosphate dehydrogenase 1) is a key enzyme involved in the de novo synthesis pathway of purine nucleotides. It catalyzes the conversion of inosine monophosphate (IMP) to xanthosine monophosphate (XMP), which is a rate-limiting step in the synthesis of guanosine monophosphate (GMP). IMPDH1 exists as a tetramer within cells and is crucial for maintaining the balance of the intracellular guanine nucleotide pool. This balance affects processes related to cell proliferation, including DNA and RNA synthesis, signal transduction, and energy transfer. Moreover, abnormal expression of IMPDH1 is associated with various diseases, including cancer and autoimmune diseases. In the retina, mutations in IMPDH1 are linked to autosomal dominant retinitis pigmentosa (adRP) type RP10. These mutations primarily affect the allosteric regulation of the enzyme rather than its catalytic activity, leading to disruptions in guanine nucleotide metabolism in the retina.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.1.1.205, IMP dehydrogenase 1, IMPD 1, IMPD1, IMPDH 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
