Human IL-4I1 ELISA Kit
Sensitivity
31.0 pg/mL
Range
125-8000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00366 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL-4I1 ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-4I1. The assay recognizes human IL-4I1. An antibody specific for human IL-4I1 has been pre-coated onto the microwells. The human IL-4I1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IL-4I1 is added to detect the captured human IL-4I1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.ntity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-4I1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 31.0 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 259307 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 81% | 76%-87% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3,319.6 | 103.7 | 3.1 |
| 2 | 20 | 737.3 | 20.1 | 2.7 |
| 3 | 20 | 167.6 | 10.6 | 6.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3,355.7 | 164.7 | 4.9 |
| 2 | 24 | 748.9 | 42.9 | 5.7 |
| 3 | 24 | 189.5 | 27.8 | 14.7 |
Background Information
Interleukin 4il (IL-4l1) is an L-phenylalanine oxidase that plays peculiar immunomodulatory roles in various types of tumors and inflammatory diseases. Its name is attributed to the early discovery of its expression in IL-4-stimulated B cells and has been implicated in the activation of B cells and T cells. IL-4I1 was discovered to be a secreted glycosylated protein, expressed by antigen-presenting cells. The IL-4I1 enzyme mainly catabolizes phenylalanine into phenylpyruvate and produces toxic derivatives, including hydrogen peroxide and ammonia. Products of IL-4I1 enzyme contain antibacterial properties which are mainly attributed to hydrogen peroxide and further amplified by the accumulation of ammonia. In addition, the IL4I1-mediated catabolite production of hydrogen peroxide has been reported to down-regulate T-cell receptor zeta expression, resulting in the inhibition of T-lymphocyte proliferation. More recently, it was discovered that secreted IL-4I1 can directly bind to human T lymphocytes, thus inhibiting effector T-cell proliferation, which is potentially independent of its enzymatic ammonia, hydrogen peroxide, and phenylpyruvate activity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL4I1,EC:1.4.3.2,FIG1,hFIG1,hIL4I1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
