Speedy™ Human IL-1R2 One-Step ELISA Kit
Sensitivity
7.0 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : SE50057
Synonyms
IL1R2, CD121 antigen-like family member B, CD121b, IL 1R 2, IL 1R beta
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-1R2 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7850 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 107% | 101%-112% |
| Human plasma | 108% | 103%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,016.6 | 18.3 | 1.8 |
| 2 | 8 | 269.4 | 6.3 | 2.3 |
| 3 | 8 | 143.8 | 3.3 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 999.3 | 34.8 | 3.5 |
| 2 | 16 | 262.6 | 10.4 | 4.0 |
| 3 | 16 | 138.0 | 6.7 | 4.9 |
Background Information
Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a receptor for the pro-inflammatory cytokines IL-1α and IL-1β. It plays a crucial role in the negative regulation of the IL-1 signaling pathway, which is involved in inflammation and immune responses. IL-1R2 also regulates IL-1 maturation and cell activation, promotes cell survival, and participates in IL-1-dependent internalization. Soluble IL-1R2 (sIL-1R2) acts as a decoy receptor by binding to IL-1α and IL-1β, preventing their interaction with IL-1R1 and IL-1RAP. This inhibits the activation of the IL-1 signaling pathway, thereby reducing inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL1R2, CD121 antigen-like family member B, CD121b, IL 1R 2, IL 1R beta |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
