Speedy™ Human IL-1R1 One-Step ELISA Kit
Sensitivity
14.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50052
Synonyms
IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6
Validation Data Gallery
Product Information
| Product name | Speedy™ Human IL-1R1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 14.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3554 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 91%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,579.3 | 57.0 | 2.2 |
| 2 | 8 | 673.0 | 10.9 | 1.6 |
| 3 | 8 | 193.4 | 5.1 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,586.0 | 63.6 | 2.5 |
| 2 | 16 | 674.9 | 13.7 | 2.0 |
| 3 | 16 | 192.2 | 8.0 | 4.2 |
Background Information
Interlukin-1 polypeptides are pleiotropic cytokines exerting diverse functions in different tissues. IL-1R1 (also known as CD121a) is a receptor for interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), and interleukin-1 receptor antagonist (IL-1Ra). Human IL-1R1 was first cloned from human T cells. This receptor contains a large cytoplasmic region and an extracellular, IL-1 binding portion composed of three immunoglobulin-like domains. IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be shed from the cell membrane as a soluble form (sIL-1R1) which can function as a soluble decoy receptor and prevent the binding of IL-1α, IL-1β, and IL-1Ra to membrane IL-1R1.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL1R1, CD121 antigen-like family member A, CD121A, D2S1473, EC:3.2.2.6 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
