Human HAPLN1 ELISA Kit
Sensitivity
0.23 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00874
Synonyms
Cartilage-link protein, Cartilage-linking protein 1, CRT1, CRTL1, Proteoglycan link protein
Validation Data Gallery
Product Information
KE00874 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HAPLN1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HAPLN1. The assay recognizes human HAPLN1. An antibody specific for human HAPLN1 has been pre-coated onto the microwells. The human HAPLN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HAPLN1 is added to detect the captured human HAPLN1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HAPLN1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.23 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1404 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 79%-87% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.25 | 1.66 | 6.31 |
| 2 | 8 | 7.09 | 0.18 | 2.61 |
| 3 | 8 | 2.46 | 0.09 | 3.76 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 26.08 | 1.72 | 6.59 |
| 2 | 16 | 7.24 | 0.26 | 3.57 |
| 3 | 16 | 2.52 | 0.11 | 4.36 |
Background Information
HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue. It is reported in the literature that it is a double band.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cartilage-link protein, Cartilage-linking protein 1, CRT1, CRTL1, Proteoglycan link protein |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
