Human GPNMB ELISA Kit
Sensitivity
0.28 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00571
Synonyms
Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB, UNQ1725/PRO9925
Validation Data Gallery
Product Information
KE00571 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GPNMB ELISA kit is to be used to detect and quantify protein levels of endogenous human GPNMB. The assay recognizes human GPNMB. An antibody specific for human GPNMB has been pre-coated onto the microwells. The human GPNMB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GPNMB is added to detect the captured human GPNMB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GPNMB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.28 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10457 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 83%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.32 | 0.47 | 1.86 |
| 2 | 8 | 6.39 | 0.25 | 3.85 |
| 3 | 8 | 3.15 | 0.15 | 4.70 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.33 | 0.59 | 2.33 |
| 2 | 16 | 6.44 | 0.26 | 4.00 |
| 3 | 16 | 3.16 | 0.15 | 4.76 |
Background Information
GPNMB (Glycoprotein non-metastatic melanoma protein B), also known as osteoactivin, is a type I transmembrane glycoprotein that plays a role in various physiological processes including cell differentiation, migration, inflammation, metabolism, and neuroprotection. It is expressed in multiple tissues such as bone, kidney, and skeletal muscle, and is implicated in cell processes like tissue regeneration and tumor progression. GPNMB has also been identified within motor neurons and radial glia, most abundantly in microglia cells, suggesting its role in inflammatory processes in the central nervous system. Its role in inflammation and tissue regeneration makes it a potential therapeutic target in diseases like atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB, UNQ1725/PRO9925 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
