Human GCSH ELISA Kit
Sensitivity
16.8 pg/mL
Range
39-2500 pg/mL
Reactivity
Human
Cat no : KE00532
Synonyms
GCSH, GCE, Glycine cleavage system H protein, mitochondrial, MMDS7, NKH
Validation Data Gallery
Product Information
KE00532 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GCSH ELISA kit is to be used to detect and quantify protein levels of endogenous human GCSH. The assay recognizes human GCSH. An antibody specific for human GCSH has been pre-coated onto the microwells. The human GCSH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GCSH is added to detect the captured human GCSH protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GCSH ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 16.8 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2653 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 90%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,151.8 | 92.3 | 8.0 |
| 2 | 8 | 283.5 | 17.3 | 6.1 |
| 3 | 8 | 154.2 | 5.7 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,172.9 | 69.9 | 6.0 |
| 2 | 16 | 294.7 | 18.9 | 6.4 |
| 3 | 16 | 147.4 | 12.6 | 8.6 |
Background Information
GCSH (glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the inner mitochondrial membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. Lipoylation of the H-protein occurs in mitochondria, which are thought to contain an activated form of lipoic acid as well as other components necessary for the transfer of lipoic acid to the protein. Defects in GCSH are a cause of non-ketotic hyperglycaemia (NKH).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GCSH, GCE, Glycine cleavage system H protein, mitochondrial, MMDS7, NKH |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
