Human FUT4 ELISA Kit
Sensitivity
0.11 ng/mL
Range
0.25-16 ng/mL
Reactivity
Human
Cat no : KE00747
Synonyms
4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-(1,3)-fucosyltransferase 4, EC:2.4.1.152, ELAM-1 ligand fucosyltransferase, ELFT
Validation Data Gallery
Product Information
KE00747 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FUT4 ELISA kit is to be used to detect and quantify protein levels of endogenous human FUT4. The assay recognizes human FUT4. An antibody specific for human FUT4 has been pre-coated onto the microwells. The human FUT4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FUT4 is added to detect the captured human FUT4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FUT4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2526 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 90%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8.60 | 0.34 | 3.97 |
| 2 | 8 | 2.25 | 0.13 | 5.79 |
| 3 | 8 | 1.03 | 0.04 | 3.46 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 8.19 | 0.49 | 6.01 |
| 2 | 16 | 2.29 | 0.12 | 5.32 |
| 3 | 16 | 1.12 | 0.10 | 8.83 |
Background Information
FUT4, also named as ELFT and FCT3A, belongs to the glycosyltransferase 10 family. FUT4 may catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. The expression of CD15 (acts as a terminal glycotope in glycoproteins and glycolipids) is directed by FUT4 in promyelocytes and monocytes. FUT4 is an antigenic epitope defined as a Lewis X carbohydrate structure is expressed on murine embryonal carcinoma cells (EC), murine ES and iPS cells, and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated ES and iPS cells and a negative surface marker for human undifferentiated ES and iPS cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in FUT4 expression. FUT4 is associated with cell adhesion, migration and differentiation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-(1,3)-fucosyltransferase 4, EC:2.4.1.152, ELAM-1 ligand fucosyltransferase, ELFT |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
