AuthentiKine™ Human FGF19 ELISA Kit
Sensitivity
1.5 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00751 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FGF19 ELISA kit is to be used to detect and quantify protein levels of endogenous human FGF19. The assay recognizes human FGF19. An antibody specific for human FGF19 has been pre-coated onto the microwells. The human FGF19 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FGF19 is added to detect the captured human FGF19 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human FGF19 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9965 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 79%-112% |
| Cell culture supernatant | 83% | 73%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 242.6 | 7.7 | 3.2 |
| 2 | 8 | 59.8 | 2.7 | 4.5 |
| 3 | 8 | 34.6 | 1.4 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 243.9 | 11.6 | 4.7 |
| 2 | 16 | 59.7 | 3.0 | 5.0 |
| 3 | 16 | 32.4 | 2.8 | 8.6 |
Background Information
Fibroblast growth factor (FGF)19 is a hormone that regulate metabolic processes particularly during feeding or starvation, thus ultimately influencing energy production. FGF19 is secreted by the intestines during feeding and negatively regulates bile acid synthesis and secretion. FGF19 is regarded as late-acting hormones because its functions are only used after insulin and glucagon have completed their actions. FGF19 shows extensively functional overlap in terms of improving glucose tolerance, insulin sensitivity, weight loss, and lipid, and energy metabolism, particularly in pathological conditions such as diabetes, obesity, metabolic syndrome, and cardiovascular and renal diseases. Most patients with these metabolic diseases exhibit reduced serum FGF19 levels, which might contribute to its etiology.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGF 19, FGF-19, Fibroblast growth factor 19, UNQ334/PRO533 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
