Human ERp57/ERp60 ELISA Kit
Sensitivity
0.09 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Cat no : KE00968
Synonyms
ERp57, PDIA3, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1
Validation Data Gallery
Product Information
KE00968 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ERp57/ERp60 ELISA kit is to be used to detect and quantify protein levels of endogenous human ERp57/ERp60. The assay recognizes human ERp57/ERp60. An antibody specific for human ERp57/ERp60 has been pre-coated onto the microwells. The human ERp57/ERp60 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ERp57/ERp60 is added to detect the captured human ERp57/ERp60 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ERp57/ERp60 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2923 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 85% | 80%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 0.36 | 10.72 | 3.33 |
| 2 | 8 | 0.16 | 2.80 | 5.66 |
| 3 | 8 | 0.03 | 1.50 | 2.32 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 0.59 | 10.45 | 5.69 |
| 2 | 16 | 0.20 | 2.77 | 7.29 |
| 3 | 16 | 0.04 | 1.48 | 2.95 |
Background Information
ERp57, also known as P58, PDIA3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, and PI-PLC, is a member of the PDI family. It participates in the oxidation, reduction, and isomerization of disulfide bonds for the correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. ERp57 was once thought to be a phospholipase; however, it has been demonstrated that this protein has protein disulfide isomerase activity. It is thought that complexes of lectins and ERp57 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERp57, PDIA3, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
