Human DSG1 ELISA Kit
Sensitivity
11.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00705
Synonyms
Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1
Validation Data Gallery
Product Information
KE00705 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DSG1 ELISA kit is to be used to detect and quantify protein levels of endogenous human DSG1. The assay recognizes human DSG1. An antibody specific for human DSG1 has been pre-coated onto the microwells. The human DSG1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DSG1 is added to detect the captured human DSG1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DSG1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1828 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 85%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,234.5 | 149.6 | 6.7 |
| 2 | 8 | 570.4 | 38.4 | 6.7 |
| 3 | 8 | 301.1 | 16.6 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,237.3 | 176.6 | 7.9 |
| 2 | 16 | 575.6 | 38.8 | 6.7 |
| 3 | 16 | 287.8 | 26.2 | 9.1 |
Background Information
Desmoglein-1 (DSG1) is a member of the desmoglein subfamily of proteins, which are key components of desmosomes, specialized cell-cell junctions that provide mechanical strength to tissues. DSG1 is crucial for maintaining the integrity of the epidermis and other epithelial tissues. Mutations in the DSG1 gene have been linked to various skin disorders, including pemphigus foliaceus, an autoimmune blistering disease, and palmoplantar keratoderma. Additionally, DSG1 expression is often downregulated in certain cancers, such as melanoma, where its loss can promote tumor cell migration. Recent studies have also shown that DSG1 expression can be affected by other genetic factors, such as PAX6 knockdown in human limbal epithelial cells. DSG1 is also involved in paracrine signaling, influencing the behavior of melanocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
