Human CLEC12A ELISA Kit
Sensitivity
5.6 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00434 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CLEC12A ELISA kit is to be used to detect and quantify protein levels of endogenous human CLEC12A. The assay recognizes human CLEC12A. An antibody specific for human CLEC12A has been pre-coated onto the microwells. The human CLEC12A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CLEC12A is added to detect the captured human CLEC12A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CLEC12A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 160364 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatant | 101% | 92%-110% |
| Cell lysate | 96% | 93%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 648.7 | 15.9 | 2.5 |
| 2 | 8 | 149.2 | 4.3 | 2.9 |
| 3 | 8 | 78.5 | 1.8 | 2.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 645.5 | 11.8 | 1.8 |
| 2 | 16 | 150.6 | 3.6 | 2.4 |
| 3 | 16 | 77.6 | 1.9 | 2.4 |
Background Information
CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily. This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated. Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLEC12A,C type lectin like molecule 1,CD371,CLL 1, |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
