Human CKAP4 ELISA Kit
Sensitivity
0.2 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00620 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CKAP4 ELISA kit is to be used to detect and quantify protein levels of endogenous human CKAP4. The assay recognizes human CKAP4. An antibody specific for human CKAP4 has been pre-coated onto the microwells. The human CKAP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CKAP4 is added to detect the captured human CKAP4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CKAP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10970 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 74%-104% |
| Cell lysate | 100% | 83%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 552.2 | 44.9 | 8.1 |
| 2 | 8 | 127.9 | 12.5 | 9.8 |
| 3 | 8 | 76.9 | 5.6 | 7.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 563.2 | 54.1 | 9.6 |
| 2 | 16 | 128.9 | 11.6 | 9.0 |
| 3 | 16 | 72.7 | 7.1 | 9.8 |
Background Information
CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation. In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2, and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
