Human CD79b ELISA Kit
Sensitivity
0.1 pg/mL
Range
3.13-200 pg/mL
Reactivity
Human
Cat no : KE00749
Synonyms
B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta, IGB
Validation Data Gallery
Product Information
KE00749 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD79b ELISA kit is to be used to detect and quantify protein levels of endogenous human CD79b. The assay recognizes human CD79b. An antibody specific for human CD79b has been pre-coated onto the microwells. The human CD79b protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD79b is added to detect the captured human CD79b protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD79b ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 974 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 84%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 112.9 | 2.9 | 2.5 |
| 2 | 8 | 28.2 | 0.7 | 2.4 |
| 3 | 8 | 14.3 | 0.5 | 3.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 108.5 | 5.2 | 4.8 |
| 2 | 16 | 29.3 | 1.3 | 4.4 |
| 3 | 16 | 14.7 | 0.6 | 4.1 |
Background Information
CD79b is a member of the Ig-Superfamily. It is a crucial component of the B cell antigen receptor (BCR) complex, essential for B cell activation and function. CD79b forms a heterodimer with CD79a (Igα) and is involved in BCR signaling, which is critical for B cell development, proliferation, and differentiation. The N-terminal tyrosine kinase structure domain of CD79b can recruit and activate downstream signaling molecules such as Syk, playing a core role in B cell signaling. Moreover, mutations in CD79b can lead to constitutive activation of downstream signaling pathways, contributing to B cell lymphoma development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta, IGB |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
