Human CD62L ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00792
Synonyms
L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc
Validation Data Gallery
Product Information
KE00792 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD62L ELISA kit is to be used to detect and quantify protein levels of endogenous human CD62L. The assay recognizes human CD62L. An antibody specific for human CD62L has been pre-coated onto the microwells. The human CD62L protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD62L is added to detect the captured human CD62L protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD62L ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6402 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 92%-124% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.78 | 0.41 | 8.54 |
| 2 | 8 | 1.19 | 0.10 | 8.60 |
| 3 | 8 | 0.65 | 0.03 | 3.99 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.63 | 0.43 | 9.26 |
| 2 | 16 | 1.19 | 0.08 | 6.69 |
| 3 | 16 | 0.65 | 0.02 | 3.21 |
Background Information
CD62L (L-selectin) is an adhesion molecule belonging to the selectin family, primarily expressed on the surface of white blood cells, and plays a role in various physiological and pathological processes, including lymphocyte homing, inflammatory responses, and immune cell positioning. It plays a crucial role in the homing process of white blood cells, helping them migrate from the bloodstream into lymphoid tissues. During inflammatory responses, CD62L mediates the initial adhesion of white blood cells to endothelial cells, facilitating their rolling and migration. Additionally, CD62L is a marker for central memory T cells, which have higher expression levels of CD62L and can rapidly relocate to lymph nodes and other sites for a swift secondary response.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
