Human CD40 ELISA Kit
Sensitivity
19.8 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00823 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD40 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD40. The assay recognizes human CD40. An antibody specific for human CD40 has been pre-coated onto the microwells. The human CD40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD40 is added to detect the captured human CD40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 19.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 958 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 96%-100% |
| Cell culture supernatant | 92% | 91%-94% |
| Saliva | 89% | 87%-91% |
| Human milk | 96% | 90%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,443.7 | 155.0 | 6.3 |
| 2 | 8 | 716.7 | 8.3 | 1.2 |
| 3 | 8 | 241.9 | 9.4 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,463.1 | 144.9 | 5.9 |
| 2 | 16 | 702.8 | 22.8 | 3.2 |
| 3 | 16 | 242.1 | 8.9 | 3.7 |
Background Information
CD40 is a member of the tumor necrosis factor receptor superfamily. It is expressed in various immune cells, including B cells, macrophages, and dendritic cells. CD40 functions as a co-stimulatory receptor that interacts with its ligand, CD40L (CD154), primarily expressed on activated CD4+ T cells. This interaction is essential for T-cell activation, immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 signaling is mediated by recruitment of TRAFs (TNF receptor-associated factors) and NF-κB activation, supplemented by pathways such as PI3K/AKT, MAPKs, and JAK3/STATs. Additionally, circulating soluble CD40 levels are elevated in patients with chronic renal failure, chronic liver diseases, Alzheimer's disease, systemic sclerosis, and hematological malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Bp50, CD40L receptor, p50, TNFRSF5 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
