Human CCR2a ELISA Kit
Sensitivity
3.5 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : KE00846
Synonyms
CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2
Validation Data Gallery
Product Information
KE00846 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CCR2a ELISA kit is to be used to detect and quantify protein levels of endogenous human CCR2a. The assay recognizes human CCR2a. An antibody specific for human CCR2a has been pre-coated onto the microwells. The human CCR2a protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CCR2a is added to detect the captured human CCR2a protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CCR2a ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 729230 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 86%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 539.9 | 17.5 | 3.2 |
| 2 | 8 | 117.9 | 3.0 | 2.6 |
| 3 | 8 | 51.6 | 2.6 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 519.1 | 30.0 | 5.8 |
| 2 | 16 | 116.1 | 4.3 | 3.7 |
| 3 | 16 | 51.2 | 3.1 | 6.1 |
Background Information
CCR2 (C-C motif chemokine receptor-2) is a transmembrane protein highly expressed in bone marrow and lymph nodes. It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. There are two isoforms of CCR2, CCR2a and CCR2b. CCR2a was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCR2, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2, CC CKR 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
