Human ARHGAP19 ELISA Kit
Sensitivity
10.8 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE01010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ARHGAP19 ELISA kit is to be used to detect and quantify protein levels of endogenous human ARHGAP19. The assay recognizes human ARHGAP19. An antibody specific for human ARHGAP19 has been pre-coated onto the microwells. The human ARHGAP19 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ARHGAP19 is added to detect the captured human ARHGAP19 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ARHGAP19 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 10.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 84986 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 105% | 98%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 967.9 | 71.4 | 7.4 |
| 2 | 8 | 238.0 | 20.9 | 8.8 |
| 3 | 8 | 122.3 | 10.9 | 8.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,037.4 | 131.6 | 12.7 |
| 2 | 16 | 260.4 | 34.6 | 13.3 |
| 3 | 16 | 129.1 | 12.0 | 9.3 |
Background Information
ARHGAP19 (Rho GTPase Activating Protein 19) is a Rho GTPase-activating protein belonging to the RhoGAP family, involved in various cytoskeleton-related events, including epithelial morphogenesis. This protein inactivates Rho protein activity by promoting GTP hydrolysis, thereby regulating cell shape and motility. In endometrial epithelial cells, the expression of ARHGAP19 is upregulated during the receptive phase, and its function is crucial for the remodeling of intercellular junctional complexes and cytoskeleton reorganization. Additionally, ARHGAP19 is involved in regulating cell polarity and apical membrane morphological changes, which facilitate embryo adhesion and invasion. Deficiencies in ARHGAP19 function may lead to loss of cell polarity, promoting the shift of non-receptive epithelial cells to a receptive phenotype.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
