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F5 Polyclonal antibody

F5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20963-1-AP

Print datasheet

Synonyms

Activated protein C cofactor, Coagulation factor V, F5, FVL, PCCF, Proaccelerin, labile factor

验证数据展示

  • WB analysis of mouse liver using 20963-1-AP

    WB analysis of mouse liver using 20963-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20963-1-AP (F5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20963-1-AP

    IHC staining of human liver cancer using 20963-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 20963-1-AP

    IHC staining of human liver cancer using 20963-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20963-1-AP (F5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

20963-1-AP targets F5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor V (proaccelerin, labile factor)
Synonyms Activated protein C cofactor, Coagulation factor V, F5, FVL, PCCF, Proaccelerin, labile factor
Calculated Molecular Weight 252 kDa
Observed Molecular Weight 145 kDa
GenBank Accession NumberNM_000130
Gene Symbol F5
Gene ID (NCBI) 2153
RRIDAB_2878773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are 13 blood coagulation factors found in the blood. There are 13 blood coagulation factors found in the blood. F5 has little or no intrinsic procoagulant activity prior to its activation through limited proteolysis by thrombin or F10a at Arg709, Arg1018 and Arg1545. The resulting F5a molecule is a heterodimer that consists of a 105 kDa heavy chain and a 71/74 kDa light chain that are noncovalently associated in a calcium-dependent manner. The intact procofactor molecule (330 kDa) as well as proteins similar in size to the factor Va light chain (74 kDa) and both light chain precursors (280 and 220 kDa) found in factor Va(IIa) were observed. And factor Xa cleaves the factor Va light chain at Arg1765 into fragments of ~30 and ~48 kDa (residues 1546-1765 and 1766-2196, respectively) in the bovine and human systems.

实验方案

Product Specific Protocols
WB protocol for F5 antibody 20963-1-APDownload protocol
IHC protocol for F5 antibody 20963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.

Authors - Sihui Yu
View Article
humanIHC

Thyroid

Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.

Authors - Kai Guo
View Article