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  • KD/KO Validated

EIF2AK1 Polyclonal antibody

EIF2AK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20499-1-AP

Print datasheet

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20499-1-AP targets EIF2AK1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen EIF2AK1 fusion protein Ag14236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_10697665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

实验方案

Product Specific Protocols
WB protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IHC protocol for EIF2AK1 antibody 20499-1-APDownload protocol
IF protocol for EIF2AK1 antibody 20499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler
humanWB

Science

Human NLRP1 is a sensor for double-stranded RNA.

Authors - Stefan Bauernfried
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
  • KD Validated
humanWB

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
  • KD Validated
humanWB

Mol Cell

Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication.

Authors - Jack Chun-Chieh Hsu
humanWB,IP

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler