- Featured Product
- KD/KO Validated
EIF2AK1 Polyclonal antibody
EIF2AK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20499-1-AP targets EIF2AK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF, IHC, WB, ELISA |
| Cited Applications | CoIP, IP, WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | EIF2AK1 fusion protein Ag14236 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 2-alpha kinase 1 |
| Synonyms | EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369 |
| Calculated Molecular Weight | 630 aa, 71 kDa |
| Observed Molecular Weight | 71 kDa |
| GenBank Accession Number | BC006524 |
| Gene Symbol | EIF2AK1 |
| Gene ID (NCBI) | 27102 |
| RRID | AB_10697665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BQI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2AK1 antibody 20499-1-AP | Download protocol |
| IHC protocol for EIF2AK1 antibody 20499-1-AP | Download protocol |
| IF protocol for EIF2AK1 antibody 20499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis
| ||
Mol Cell Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication. | ||
Nat Commun Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis. |
