Validation Data Gallery
Product Information
Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag.
| Applications | Immunoprecipitation: use ~ 5 μg EGFP per 25 μL GFP-Trap® bead slurry SDS-PAGE: use ~ 1 μg EGFP per lane Western blot: use ~ 25 ng - 100 ng EGFP per lane ELISA: coat ~ 1ng - 50 ng per well (96-well plate) Other in vitro assays: as a fluorescent standard and for calibration |
| Molecular Weight | 28.0 kDa (including the polyhistidine-tag) |
| Form | Liquid |
| Size | 250 µL |
| Concentration | 1 g/L |
| Conjugate | Unconjugated |
| Purity | > 80% by SDS-PAGE |
| Source | Escherichia coli |
| Storage Buffer | 0.09% sodium azide |
| Storage Condition | Shipped at +4°C. Store at +4°C short term (up to 1 week). Store at -20°C or -80°C long term. Avoid freeze-thaw cycles. Stable for one year. |
Documentation
| SDS |
|---|
| EGFP_SDS_EGFP, recombinant purified protein (EN) |
| Datasheet |
|---|
| EGFP, recombinant purified protein Datasheet |
Publications
| Application | Title |
|---|---|
Cell Cellular Control of Viscosity Counters Changes in Temperature and Energy Availability. | |
Nat Cell Biol Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis | |
Trends Biotechnol Engineered thermoswitch in the baculovirus expression vector system for production of virus-like particle vaccines with minimized baculovirus contaminants | |
Trends Biotechnol Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles. | |
Microsyst Nanoeng Employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices |
