验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.06 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-FcA98161 targets CD6 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1401 Product name: Recombinant Mouse CD6 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 17-396 aa of NM_009852.3 Sequence: GLPSPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCAAHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQDQECSSDRRLVWVTCAENQAVRLVDGSSRCAGRVEMLEHGEWGTVCDDTWDLQDAHVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGIDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCRSLGCGSAVARPRGLPHSLDGRMYYSCKGQEPALSTCSWRFNNSNLCSQSRAARVVCSGSQRHLNLSTSEVPSRVPVTIESSVPVSVKDKDSQG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD6 antigen |
| 别名 | T-cell differentiation antigen CD6 |
| 计算分子量 | 72kDa |
| GenBank蛋白编号 | NM_009852.3 |
| 基因名称 | Cd6 |
| Gene ID (NCBI) | 12511 |
| RRID | AB_3749803 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q61003-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD6 antibody PE-FcA98161 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
