验证数据展示
经过测试的应用
| Positive FC detected in | rabbit splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PY7-98349 targets CD3 in FC applications and shows reactivity with rabbit samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | rabbit |
| 免疫原 |
CatNo: Eg3525 Product name: recombinant rabbit CD3E+CD3G共表达 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Sequence: / 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | T-cell surface glycoprotein CD3 epsilon chain |
| 别名 | CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e |
| 基因名称 | CD3E |
| Gene ID (NCBI) | 100008616 |
| RRID | AB_3750006 |
| 偶联类型 | PE-Cyanine7 Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 778 nm |
| 激发激光 | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE-Cyanine7 CD3 antibody PY7-98349 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
