验证数据展示
经过测试的应用
| Positive FC detected in | mouse bone marrow cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| FC | See 10 publications below |
产品信息
APC-65055 targets CD11b in FC applications and shows reactivity with human, mouse, rabbit, cynomolgus monkey, guinea pig samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | FC |
| 经测试反应性 | human, mouse, rabbit, cynomolgus monkey, guinea pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | integrin alpha M |
| 别名 | CR3, CR3A, integrin alpha M, Itgam, Ly 40 |
| 计算分子量 | 127 kDa |
| GenBank蛋白编号 | BC156991 |
| 基因名称 | CD11b |
| Gene ID (NCBI) | 16409 |
| RRID | AB_2882958 |
| 偶联类型 | APC Fluorescent Dye |
| 最大激发/发射波长 | 650 nm / 660 nm |
| 激发激光 | Red Laser (633 nm) |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | E9Q604 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD11b antibody APC-65055 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Nat Cell Biol WDR4 drives tumour-associated macrophage reprogramming and tumour progression via selective translation and membrane cholesterol efflux | ||
J Nanobiotechnology PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer | ||
Adv Sci (Weinh) Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer | ||
Adv Sci (Weinh) A Porphyrin Nanomaterial for Photoimmunotherapy for Treatment of Melanoma | ||
Cell Death Dis EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis |
