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验证数据展示

  • WB analysis of NCI-H1299 using 14787-1-AP

    WB analysis of NCI-H1299 using 14787-1-AP

    WB result of CBS antibody (14787-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBS transfected NCI-H1299 cells.

  • WB analysis using 14787-1-AP

    WB analysis using 14787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14787-1-AP

    WB analysis using 14787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14787-1-AP (CBS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 14787-1-AP

    IP experiment of mouse kidney using 14787-1-AP

    IP result of anti-CBS (IP:14787-1-AP, 3ug; Detection:14787-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • IHC staining of human pancreas cancer using 14787-1-AP

    IHC staining of human pancreas cancer using 14787-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 14787-1-AP

    IHC staining of human pancreas cancer using 14787-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14787-1-AP (CBS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14787-1-AP

    IF Staining of HeLa using 14787-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of MCF-7 using 14787-1-AP

    IF Staining of MCF-7 using 14787-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse kidney using 14787-1-AP

    IHC staining of mouse kidney using 14787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14787-1-AP (CBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14787-1-AP

    IF Staining of HeLa using 14787-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CBS antibody (14787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

CBS Polyclonal antibody

CBS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14787-1-AP

产品说明书

引用文献(159)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Beta thionase, Beta-thionase, cystathionine beta synthase, Cystathionine beta-synthase, EC:4.2.1.22

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse colon tissue, NCI-H1299 cells, rat kidney tissue, HepG2 cells, LNCaP cells, MCF-7 cells, SKOV-3 cells, THP-1 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14787-1-AP targets CBS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, canine, bovine
免疫原

CatNo: Ag6437

Product name: Recombinant human CBS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 206-551 aa of BC000440

Sequence: VAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cystathionine-beta-synthase
别名 Beta thionase, Beta-thionase, cystathionine beta synthase, Cystathionine beta-synthase, EC:4.2.1.22
计算分子量 61 kDa
观测分子量 61-63 kDa
GenBank蛋白编号BC000440
基因名称 CBS
Gene ID (NCBI) 875
RRIDAB_2070970
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

实验方案

Product Specific Protocols
IF protocol for CBS antibody 14787-1-APDownload protocol
IHC protocol for CBS antibody 14787-1-APDownload protocol
IP protocol for CBS antibody 14787-1-APDownload protocol
WB protocol for CBS antibody 14787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Signal Transduct Target Ther

B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis

Authors - Xing Du
View Article
mouseWB

Cell Metab

Mitochondrial dysfunction reveals H(2)S-mediated synaptic sulfhydration as a potential mechanism for autism-associated social defects.

Authors - Panpan Xian
View Article
humanWB

Cancer Res

PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer

Authors - Takehiro Yamamoto
View Article
humanWB,IHC

Cancer Res

Upregulation of Cystathionine-β-synthase in Colonic Epithelia Reprograms Metabolism and Promotes Carcinogenesis.

Authors - Ches'Nique M Phillips
View Article
canineIF

Nat Commun

Remote ischemic conditioning counteracts the intestinal damage of necrotizing enterocolitis by improving intestinal microcirculation.

Authors - Yuhki Koike
View Article
mouseWB

J Adv Res

Endogenous hydrogen sulfide improves vascular remodeling through PPARδ/SOCS3 signaling.

Authors - Danyang Tian
View Article
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