ChromoTek pSpot2 vector

The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

Vector type

bacterial expression vector

Promoter

T7

Reporter

Spot-Tag® C-terminal

Cat No : ev-2

Synonyms

pSpot2 vector, Spot vector



Product Information

The plasmid pSpot2 is an expression vector for Spot-Tag® fusion proteins in E. coli. After cloning the protein of interest (POI) into the multiple cloning site (MCS) provided by pSpot2, the Spot-Tag® (sequence: PDRVRAVSHWSS) will be fused to the C-terminus of the POI.

ApplicationsProtein expression
Vector typebacterial expression vector
PromoterT7
ReplicationpBR322
ReporterSpot-Tag® (PDRVRAVSHWSS) C-terminal
Selectionkanamycin

Documentation

SDS
SDS.pdf
Datasheet
ev-2.pdf

Publications

ApplicationTitle

Cells

The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.

Authors - Mohamed Ibrahem Elhawy

Int J Mol Sci

Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice

Authors - Nour Ahmad-Mansour