Validation Data Gallery
![HeLa cells transiently expressing Lamin-Chromobody?-mNeonGreen were immunostained with mNeonGreen recombinant antibody, VHH-mouse IgG1 Fc fusion [CTK0203] (nfms-20, 1:500) and Nano-Secondary? alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor? 647 [CTK0103, CTK0104] (sms1AF647-1, 1:500). Scale bar, 10 μM](/products/pictures/nfms-1.jpg "HeLa cells transiently expressing Lamin-Chromobody?-mNeonGreen were immunostained with mNeonGreen recombinant antibody, VHH-mouse IgG1 Fc fusion [CTK0203] (nfms-20, 1:500) and Nano-Secondary? alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor? 647 [CTK0103, CTK0104] (sms1AF647-1, 1:500). Scale bar, 10 μM")
Product Information
Mouse monoclonal antibody to mNeonGreen. Recombinant fusion of anti-mNeonGreen VHH [CTK0203] and mouse IgG1 Fc domain.
| Applications | IF |
| Host/IsoType | Mouse/IgG1 |
| Species Specificity | Recombinant protein from Branchiostoma lanceolatum |
| Specificity/Target | mNeonGreen fluorescent protein |
| Conjugate | Unconjugated |
| Recommended Dilution | IF/ICC: 1:400 - 1:2,000 |
| GenBank Accession Number | KC295282 |
| Purification Method | Recombinantly expressed and purified by IMAC |
| Type | Primary antibody; chimeric fusion of Nanobody (VHH) and mouse IgG1 Fc domain |
| Class | Recombinant |
| Format | Recombinant monoclonal mouse antibody |
| Immunogen | Purified recombinant mNeonGreen [Branchiostoma lanceolatum] |
| Clonality | Monoclonal |
| Clone No. | CTK0203 |
| Calculated MW | 79.3 kDa |
| Form | Buffered aqueous solution |
| RRID | AB_2889368 |
| Storage Buffer | PBS, 10% glycerol, Preservative: 0.02 % sodium azide, safety datasheet (SDS): sodium azide |
| Storage Condition | Shipped on ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Size | 20 µL; 100 µL |
Publications
| Application | Title |
|---|---|
PLoS One Analysis of the Leishmania mexicana promastigote cell cycle using imaging flow cytometry provides new insights into cell cycle flexibility and events of short duration |