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Recombinant Human TL1A/TNFSF15 protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 2-9 ng/mL

Cat no : Eg32092

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Synonyms

TNFSF15, TNFSF 15, Tumor necrosis factor ligand superfamily member 15, Tumor necrosis factor ligand superfamily member 15, membrane form, Tumor necrosis factor ligand superfamily member 15, secreted form

验证数据展示

  • Purity of Recombinant Human TL1A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TL1A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.

    Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Immobilized Human TL1A (His tag) at 0.5 μg/mL (100 μL/well) can bind Human DcR3 (His tag) with a linear range of 2-9 ng/mL.
来源 HEK293-derived Human TL1A protein Leu72-Leu251 (Accession# O95150-1) with a His tag at the N-terminus.
基因ID 9966
蛋白编号 O95150-1
预测分子量 21.3 kDa
SDS-PAGE 25-29 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB. TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines. Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing. In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro).

参考文献:

1.Haridas V. et al. (1999). Oncogene. 18(47):6496-504. 
2.Migone TS. et al. (2002). Immunity. 16(3):479-92
3. Chew LJ. et al. (2002). FASEB J. 16(7):742-4.
4. Zhai Y. et al. (1999). FASEB J. 13(1):181-9.