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验证数据展示

  • WB analysis of SGC-7901 using 28492-1-AP

    WB analysis of SGC-7901 using 28492-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 28492-1-AP (filamin-c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 28492-1-AP

    IHC staining of human colon using 28492-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 28492-1-AP (FLNC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Filamin-C Polyclonal antibody

Filamin-C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28492-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FLNC, FLN2, FilaminC, Filamin C, Filamin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28492-1-AP targets Filamin-C in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Filamin-C fusion protein Ag29328 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 filamin C, gamma (actin binding protein 280)
别名 FLNC, FLN2, FilaminC, Filamin C, Filamin 2
计算分子量 291 kDa
观测分子量 280-300 kDa
GenBank蛋白编号NM_001458
基因名称 FLNC
Gene ID (NCBI) 2318
RRIDAB_2918171
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14315
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Filamin C (FLNC; also known as γ-FLN; ABP-L; FLN2), a muscle-specific filamin and a large actin-cross-linking protein. Human filamins are ~280 kDa homodimers, each monomer consisting of an N-terminal actin-binding domain followed by 24 immunoglobulin (Ig)-like domains (d1 to d24), the last of which mediates dimerization. FLNC is specifically expressed in cardiomyocytes and skeletal myocytes and is involved in the maintenance of structural integrity. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. ( PMID: 25577646, PMID: 30867563, PMID: 31131323)

实验方案

Product Specific Protocols
WB protocol for Filamin-C antibody 28492-1-APDownload protocol
IHC protocol for Filamin-C antibody 28492-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions

Authors - Ilenia Matera
View Article