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验证数据展示

  • WB analysis using 21583-1-AP

    WB analysis using 21583-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21583-1-AP (ZNF75D antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 21583-1-AP

    WB analysis of RAW 264.7 using 21583-1-AP

    WB result of ZNF75D antibody (21583-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF75D transfected RAW 264.7 cells.

  • Featured Product
  • KD/KO验证

ZNF75D Polyclonal antibody

ZNF75D Polyclonal Antibody for WB, ELISA
Cat No. 21583-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

D8C6, Zinc finger protein 75, zinc finger protein 75D, Zinc finger protein 82, ZNF75, ZNF75D, ZNF82

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21583-1-AP targets ZNF75D in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 ZNF75D fusion protein Ag16139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 75D
别名 D8C6, Zinc finger protein 75, zinc finger protein 75D, Zinc finger protein 82, ZNF75, ZNF75D, ZNF82
计算分子量 510 aa, 59 kDa
观测分子量35 kDa
GenBank蛋白编号BC109100
基因名称 ZNF75D
Gene ID (NCBI) 7626
RRIDAB_3085661
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51815
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF75D (zinc finger protein 75D), also known as ZNF75. It is expected to be located in nucleus and ubiquitous expression in prostate and adrenal. The calculated molecular weight of METAP1 is 60 kDa. The gene encodes a protein that likely functions as a transcription factor. ZNF75D belongs to the ZNF75 family, includes an N-terminal SCAN domain, a KRAB box, and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16, while pseudogenes have been identified on chromosomes 11 and 12 (PMID: 8661144). It may be involved in transcriptional regulation.

实验方案

Product Specific Protocols
WB protocol for ZNF75D antibody 21583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols