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验证数据展示

  • WB analysis using 86430-1-RR

    WB analysis using 86430-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86430-1-RR

    WB analysis using 86430-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86430-1-RR

    Affinity and Kinetic Characterization of 86430-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86430-1-RR against Human ZMYND10 were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

ZMYND10 Recombinant monoclonal antibody

ZMYND10 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86430-1-RR

产品说明书

CloneNo. 251276H4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue, human testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86430-1-RR targets ZMYND10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5808

Product name: Recombinant human ZMYND10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-440 aa of BC033732

Sequence: MGDLELLLPGEAEVLVRGLRSFPLREMGSEGWNQQHENLEKLNMQAILDATVSQGEPIQELLVTHGKVPTLVEELIAVEMWKQKVFPVFCRVEDFKPQNTFPIYMVVHHEASIINLLETVFFHKEVCESAEDTVLDLVDYCHRKLTLLVAQSGCGGPPEGEGSQDSNPMQELQKQAELMEFEIALKALSVLRYITDCVDSLSLSTLSRMLSTHNLPCLLVELLEHSPWSRREGGKLQQFEGSRWHTVAPSEQQKLSKLDGQVWIALYNLLLSPEAQARYCLTSFAKGRLLKLRAFLTDTLLDQLPNLAHLQSFLAHLTLTETQPPKKDLVLEQIPEIWERLERENRGKWQAIAKHQLQHVFSPSEQDLRLQARRWAETYRLDVLEAVAPERPRCAYCSAEASKRCSRCQNEWYCCRECQVKHWEKHGKTCVLAAQGDRAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger, MYND-type containing 10
别名 BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC033732
基因名称 ZMYND10
Gene ID (NCBI) 51364
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75800
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).

实验方案

Product Specific Protocols
WB protocol for ZMYND10 antibody 86430-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols