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验证数据展示

  • WB analysis of mouse brain using 25742-1-AP

    WB analysis of mouse brain using 25742-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25742-1-AP (ZMYM3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 25742-1-AP

    IP experiment of K-562 using 25742-1-AP

    IP result of anti-ZMYM3 (IP:25742-1-AP, 4ug; Detection:25742-1-AP 1:300) with K-562 cells lysate 3600ug.

ZMYM3 Polyclonal antibody

ZMYM3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25742-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

别名

DXS6673E, KIAA0385, MYM, XFIM, Zinc finger MYM type protein 3, Zinc finger protein 261, zinc finger, MYM type 3, ZMYM3, ZNF198L2, ZNF261

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ZMYM3 Ready-To-Use IHC Kit. ZMYM3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25742-1-AP targets ZMYM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag22701

Product name: Recombinant human ZMYM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC013009

Sequence: MDPSDFPSPFDPLTLPEKPLAGDLPVDMEFGEDLLESQTAPTRGWAPPGPSPSSGALDLLDTPAGLEKDPGVLDGATELLGLGGLLYKAPSPPEVDHGPEGTLAWDAGDQTLEPGPGGQTPEVVPPDPGAGANSCSPEGLLEPLAPDSPITLQSPHIEEEETTSIATARRGSPGQEEELPQGQPQSPNAPPSPSVGETLGDGINSSQTKPGGSSPPAHPSLPGDGLTAKASEKPPERKRSERVRRAEPPKPEVVDSTESIPVSDEDSDAMVDDPNDEDFVPFRPRRSPRMSLRSSVSQRAGRSAVGTKMTCAHCRTP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, MYM-type 3
别名 DXS6673E, KIAA0385, MYM, XFIM, Zinc finger MYM type protein 3, Zinc finger protein 261, zinc finger, MYM type 3, ZMYM3, ZNF198L2, ZNF261
计算分子量 1370 aa, 152 kDa
观测分子量 190-200 kDa
GenBank蛋白编号BC013009
基因名称 ZMYM3
Gene ID (NCBI) 9203
RRIDAB_2880221
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14202
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMYM3, also named as DXS6673E, KIAA0385, and ZNF261, is a 1370 amino acid protein, which may be a component of a BHC histone deacetylase complex. ZMYM3 is most abundant in brain, moderate in muscle and heart, low in other tissues except placenta. ZMYM3 plays a role in the regulation of cell morphology and cytoskeletal organization.

实验方案

Product Specific Protocols
IP protocol for ZMYM3 antibody 25742-1-APDownload protocol
WB protocol for ZMYM3 antibody 25742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IHC

Nat Commun

Transposable elements-mediated recruitment of KDM1A epigenetically silences HNF4A expression to promote hepatocellular carcinoma

Authors - Tiantian Jing
View Article
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