验证数据展示
经过测试的应用
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ZFYVE26 Ready-To-Use IHC Kit. ZFYVE26 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83215-4-RR targets ZFYVE26 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | zinc finger, FYVE domain containing 26 |
| 别名 | SPG15, Spastizin, KIAA0321, 230493D3 |
| 计算分子量 | 285 kDa |
| GenBank蛋白编号 | NM_015346 |
| 基因名称 | ZFYVE26 |
| Gene ID (NCBI) | 23503 |
| RRID | AB_3670900 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q68DK2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZFYVE26 is a large protein of 2539 amino acid residues and contains an FYVE domain that confers to the protein binding affinity to the membrane-associated phosphatidylinositol 3-phosphate (PtdIns3P). ZFYVE26 has a diffuse cytoplasmic distribution and partially colocalizes with early endosomes, the endoplasmic reticulum, microtubules, and vesicles. It also localizes to the midbody during cytokinesis (PMID: 24284334).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZFYVE26 antibody 83215-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
