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验证数据展示

  • WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    WB analysis using 85154-4-RR (same clone as 85154-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85154-4-RR (ZFP36L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85154-4-RR (same clone as 85154-4-PBS)

    IF Staining of HepG2 using 85154-4-RR (same clone as 85154-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZFP36L2 antibody (85154-4-RR, Clone: 242776C5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85154-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85154-4-RR

    Affinity and Kinetic Characterization of 85154-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85154-4-RR against Human ZFP36L2 were performed. The affinity constant is 2.38 nM.

ZFP36L2 Recombinant monoclonal antibody, PBS Only

ZFP36L2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85154-4-PBS

产品说明书

CloneNo. 242776C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, monkey

应用

WB, IF/ICC, Indirect ELISA

别名

BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85154-4-PBS targets ZFP36L2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, monkey samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, monkey
免疫原

CatNo: Ag13515

Product name: Recombinant human ZFP36L2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 155-497 aa of BC005010

Sequence: KTELCRPFEESGTCKYGEKCQFAHGFHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNADERRPAPSGGASGDLRAFGTRDALHLGFPREPRPKLHHSLSFSGFPSGHHQPPGGLESPLLLDSPTSRTPPPPSCSSASSCSSSASSCSSASAASTPSGAPTCCASAAAAAAAALLYGTGGAEDLLAPGAPCAACSSASCANNAFAFGPELSSLITPLAIQTHNFAAVAAAAYYRSQQQQQQQQQQGLAPPAQPPAPPSATLPAGAAAPPSPPFSFQLPRRLSDSPVFDAPPSPPDSLSDRDSYLSGSLSSGSLSGSESPSLDPGRRLPIFSRLSISDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 36, C3H type-like 2
别名 BRF2, Butyrate response factor 2, EGF response factor 2, EGF-response factor 2, ERF 2
计算分子量 51 kDa
观测分子量60 kDa
GenBank蛋白编号BC005010
基因名称 ZFP36L2
Gene ID (NCBI) 678
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP47974
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZFP36L2 (Zinc finger protein 36 like 2) is an RNA-binding protein belonging to the ZFP36 family, which regulates gene expression at the post-transcriptional level by binding and destabilizing certain AU-rich elements (ARE) located in the 3′ untranslated regions (3′UTRs) of mRNA (PMID: 29518627). Moreover, ZFP36L2 is a key protein that controls S-phase progression in the case of genome instability (PMID: 29449217).

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