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验证数据展示

  • FC experiment of MCF-7 using 82912-3-RR

    FC experiment of MCF-7 using 82912-3-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of A431 using 82912-3-RR

    FC experiment of A431 using 82912-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human ZC3H11A (82912-3-RR, Clone:230191B4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82912-3-RR

    Affinity and Kinetic Characterization of 82912-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82912-3-RR against Human ZC3H11A were performed. The affinity constant is below 1 pM.

ZC3H11A Recombinant antibody

ZC3H11A Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 82912-3-RR

产品说明书

CloneNo. 230191B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191B4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA431 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82912-3-RR targets ZC3H11A in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZC3H11A fusion protein Ag23424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger CCCH-type containing 11A
别名 Zinc finger CCCH domain-containing protein 11A, ZC3HDC11A, KIAA0663, 230191B4
GenBank蛋白编号BC014268
基因名称 ZC3H11A
Gene ID (NCBI) 9877
RRIDAB_3670645
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.