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验证数据展示

  • IHC staining of human liver cancer using 81161-3-RR (same clone as 81161-3-PBS)

    IHC staining of human liver cancer using 81161-3-RR (same clone as 81161-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81161-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZAK antibody included
  • IF Staining of HepG2 using 81161-3-RR (same clone as 81161-3-PBS)

    IF Staining of HepG2 using 81161-3-RR (same clone as 81161-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ZAK antibody (81161-3-RR, Clone: 250559E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81161-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81161-3-RR

    Affinity and Kinetic Characterization of 81161-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81161-3-RR against Human ZAK were performed. The affinity constant is 2.57 nM.

    {{ptg:ProductFeaturesInfo}}

ZAK Recombinant monoclonal antibody, PBS Only

ZAK Uni-rAb® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 81161-3-PBS

产品说明书

CloneNo. 250559E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ZAK Ready-To-Use IHC Kit. ZAK primary antibody included.

产品信息

81161-3-PBS targets ZAK in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30599

Product name: Recombinant human ZAK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 223-325 aa of BC001401

Sequence: ERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sterile alpha motif and leucine zipper containing kinase AZK
别名 AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase
计算分子量 91 kDa
GenBank蛋白编号BC001401
基因名称 ZAK
Gene ID (NCBI) 51776
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NYL2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.