验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22407 Product name: Recombinant human YTHDF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 284-351 aa of BC052970 Sequence: SVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | YTH domain family, member 3 |
| 别名 | YTH domain-containing family protein 3, DF3, 242149F3 |
| 计算分子量 | 585 aa, 64 kDa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | BC052970 |
| 基因名称 | YTHDF3 |
| Gene ID (NCBI) | 253943 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q7Z739 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF3 antibody 84709-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
