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验证数据展示

  • WB analysis using 83970-1-RR

    WB analysis using 83970-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83970-1-RR

    FC experiment of HepG2 using 83970-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of HeLa using 83970-1-RR

    WB analysis of HeLa using 83970-1-RR

    WB result of YTHDC2 antibody (83970-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells.

  • IHC staining of rat testis using 83970-1-RR

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • IHC staining of rat testis using 83970-1-RR

    IHC staining of rat testis using 83970-1-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YTHDC2 antibody included
  • Affinity and Kinetic Characterization of 83970-1-RR

    Affinity and Kinetic Characterization of 83970-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83970-1-RR against Human YTHDC2 were performed. The affinity constant is 0.378 nM.

  • Featured Product
  • KD/KO验证

YTHDC2 Recombinant monoclonal antibody

YTHDC2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83970-1-RR

产品说明书

CloneNo. 241123A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

241123A9, 3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDC2 Ready-To-Use IHC Kit. YTHDC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83970-1-RR targets YTHDC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26690

Product name: Recombinant human YTHDC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1131-1430 aa of BC137285

Sequence: HSLFLRRMRAPSKPWSQVDEATIRAIIAVLSTEEQSAGLQQPSGIGQRPRPMSSEELPLASSWRSNNSRKSSADTEFSDECTTAERVLMKSPSPALHPPQKYKDRGILHPKRGTEDRSDQSSLKSTDSSSYPSPCASPSPPSSGKGSKSPSPRPNMPVRYFIMKSSNLRNLEISQQKGIWSTTPSNERKLNRAFWESSIVYLVFSVQGSGHFQGFSRMSSEIGREKSQDWGSAGLGGVFKVEWIRKESLPFQFAHHLLNPWNDNKKVQISRDGQELEPLVGEQLLQLWERLPLGEKNTTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain containing 2
别名 241123A9, 3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2
观测分子量160 kDa
GenBank蛋白编号BC137285
基因名称 YTHDC2
Gene ID (NCBI) 64848
RRIDAB_3671546
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H6S0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

实验方案

Product Specific Protocols
IHC protocol for YTHDC2 antibody 83970-1-RRDownload protocol
WB protocol for YTHDC2 antibody 83970-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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