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验证数据展示

  • WB analysis using 84902-1-RR

    WB analysis using 84902-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 84902-1-RR

    IP experiment of Jurkat using 84902-1-RR

    IP result of anti-XPR1 (IP:84902-1-RR, 4ug; Detection:84902-1-RR 1:1000) with Jurkat cells lysate 1800 ug.

  • Affinity and Kinetic Characterization of 84902-1-RR

    Affinity and Kinetic Characterization of 84902-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

XPR1 Recombinant monoclonal antibody

XPR1 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 84902-1-RR

产品说明书

CloneNo. 241788A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

241788A7, Phosphate exporter SLC53A1, Protein SYG1 homolog, SLC53A1, Solute carrier family 53 member 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84902-1-RR targets XPR1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5373

Product name: Recombinant human XPR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC041142

Sequence: MKFAEHLSAHITPEWRKQYIQYEAFKDMLYSAQDQAPSVEVTDEDTVKRYFAKFEEKFFQTCEKELAKINTFYSEKLAEAQRRFATLQNELQSSLDAQKESTGVTTLRQRRKPVFHLSHEERVQHRNIKDLKLAFSEFYLSLILLQNYQNLNFTGFRKILKKHDKILETSRGADWRVAHVEVAPFYTCKKINQLISETEAVVTNELEDGDRQKAMKRLRVPPLGAAQPAPAWTT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 xenotropic and polytropic retrovirus receptor
别名 241788A7, Phosphate exporter SLC53A1, Protein SYG1 homolog, SLC53A1, Solute carrier family 53 member 1
计算分子量 696 aa, 82 kDa
观测分子量74 kDa
GenBank蛋白编号BC041142
基因名称 XPR1
Gene ID (NCBI) 9213
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBH6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.

实验方案

Product Specific Protocols
WB protocol for XPR1 antibody 84902-1-RRDownload protocol
IP protocol for XPR1 antibody 84902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols