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验证数据展示

  • WB analysis of HepG2 using 85886-4-RR

    WB analysis of HepG2 using 85886-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 85886-4-RR (WNK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85886-4-RR

    Affinity and Kinetic Characterization of 85886-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85886-4-RR against Human WNK4 were performed. The affinity constant is 0.926 nM.

WNK4 Recombinant antibody

WNK4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85886-4-RR

产品说明书

CloneNo. 250156A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, A375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85886-4-RR targets WNK4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 WNK4 fusion protein Ag17773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 WNK lysine deficient protein kinase 4
别名 EC:2.7.11.1, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Serine/threonine-protein kinase WNK4
计算分子量 1243 aa, 135 kDa
观测分子量 220 kDa
GenBank蛋白编号BC136664
基因名称 WNK4
Gene ID (NCBI) 65266
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96J92
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNK4 (With No Lysine Kinase 4) is a member of the WNK family of serine/threonine protein kinases. It plays a crucial role in regulating electrolyte balance and blood pressure by modulating the activity of ion transporters in the kidney. WNK4 is primarily involved in the regulation of sodium and potassium homeostasis. It achieves this by phosphorylating and activating the kinases SPAK and OSR1, which in turn regulate the activity of the sodium-chloride cotransporter (NCC) in the distal convoluted tubule of the kidney. Additionally, by promoting sodium reabsorption and potassium secretion, WNK4 contributes to the regulation of blood pressure. Increased activity of WNK4 can lead to hypertension. (PMID: 24655003; 33491560)

实验方案

Product Specific Protocols
WB protocol for WNK4 antibody 85886-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols