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验证数据展示

  • WB analysis using 84714-2-RR

    WB analysis using 84714-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84714-2-RR (WASP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84714-2-RR

    Affinity and Kinetic Characterization of 84714-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84714-2-RR against Human WASP were performed. The affinity constant is 1.88 nM.

WASP Recombinant antibody

WASP Recombinant Antibody for WB, ELISA
Cat No. 84714-2-RR

产品说明书

CloneNo. 242074E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, TF-1 cells, HL-60 cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy WAS Ready-To-Use IHC Kit. WAS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84714-2-RR targets WASP in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 WASP fusion protein Ag1444 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
别名 Wiskott-Aldrich syndrome protein, WAS, Actin nucleation-promoting factor WAS, 242074E4
计算分子量 53 kDa
观测分子量 58 kDa
GenBank蛋白编号BC012738
基因名称 WASP
Gene ID (NCBI) 7454
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.

实验方案

Product Specific Protocols
WB protocol for WASP antibody 84714-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols