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TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C3

Cat No : 67952-1-Ig

Print datasheet

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

验证数据展示

  • WB analysis using 67952-1-Ig

    WB analysis using 67952-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of LNCaP using 67952-1-Ig

    IF Staining of LNCaP using 67952-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, 4T1 cells, human placenta tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF detected inLNCaP cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67952-1-Ig targets TrpRS in WB, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, rat, mouse
Immunogen TrpRS fusion protein Ag31430 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tryptophanyl-tRNA synthetase
Synonyms GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
Calculated Molecular Weight 471 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017489
Gene Symbol WARS
Gene ID (NCBI) 7453
RRIDAB_2918704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23381
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

实验方案

Product Specific Protocols
WB protocol for TrpRS antibody 67952-1-IgDownload protocol
IF protocol for TrpRS antibody 67952-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols