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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

    WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

    IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

TrpRS Monoclonal antibody, PBS Only

TrpRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67952-1-PBS

产品说明书

CloneNo. 1C6C3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.

产品信息

67952-1-PBS targets TrpRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag31430

Product name: Recombinant human WARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tryptophanyl-tRNA synthetase
别名 GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
计算分子量 471 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017489
基因名称 WARS1
Gene ID (NCBI) 7453
RRIDAB_2918704
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23381
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

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