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CoraLite® Plus 488-conjugated TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6C3

Cat No : CL488-67952

Print datasheet

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

验证数据展示

  • FC experiment of HeLa using CL488-67952

    FC experiment of HeLa using CL488-67952

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TrpRS (CL488-67952, Clone:1C6C3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-67952

    IF Staining of MCF-7 using CL488-67952

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 TrpRS antibody (CL488-67952, Clone: 1C6C3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67952-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67952 targets TrpRS in IF, FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, rat, mouse
Immunogen TrpRS fusion protein Ag31430 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tryptophanyl-tRNA synthetase
Synonyms GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS
Calculated Molecular Weight 471 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017489
Gene Symbol WARS
Gene ID (NCBI) 7453
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23381
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
FC protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
Standard Protocols
Click here to view our Standard Protocols