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验证数据展示

  • IF Staining of MCF-7 using CL488-67952

    IF Staining of MCF-7 using CL488-67952

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 TrpRS antibody (CL488-67952, Clone: 1C6C3 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67952

    FC experiment of HeLa using CL488-67952

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TrpRS (CL488-67952, Clone:1C6C3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated TrpRS Monoclonal antibody

TrpRS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67952

产品说明书

CloneNo. 1C6C3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

WARS, 1C6C3, EC:6.1.1.2, GAMMA 2, hWRS

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy WARS Ready-To-Use IHC Kit. WARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67952-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67952 targets TrpRS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31430

Product name: Recombinant human WARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tryptophanyl-tRNA synthetase
别名 WARS, 1C6C3, EC:6.1.1.2, GAMMA 2, hWRS
计算分子量 471 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017489
基因名称 WARS1
Gene ID (NCBI) 7453
RRIDAB_2934596
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP23381
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
IF protocol for CL Plus 488 TrpRS antibody CL488-67952Download protocol
Standard Protocols
Click here to view our Standard Protocols
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