验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A549 |
| Positive IHC detected in | Lung cancer tissue, human lung cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
65039-1-Ig targets VIM in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vimentin |
| 别名 | VIM |
| 计算分子量 | 54 kDa |
| GenBank蛋白编号 | BC066956 |
| 基因名称 | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Other |
| UNIPROT ID | P08670 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |