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验证数据展示

  • WB analysis using 13303-1-AP

    WB analysis using 13303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13303-1-AP (VASH2 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 13303-1-AP

    IF Staining of A549 using 13303-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using VASH2 antibody (13303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human stomach cancer using 13303-1-AP

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 13303-1-AP

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 13303-1-AP

    IHC staining of human stomach cancer using 13303-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13303-1-AP (VASH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VASH2 Polyclonal antibody

VASH2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13303-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, BxPC-3 cells, L02 cells, SW 1990 cells, PANC-1 cells, PC-12 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13303-1-AP targets VASH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性rat
免疫原 VASH2 fusion protein Ag4121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vasohibin 2
别名 Vasohibin-like protein, Vasohibin-2, VASHL, Tubulinyl-Tyr carboxypeptidase 2, EC:3.4.17.17
计算分子量 355 aa, 40 kDa
观测分子量30-34 kDa
GenBank蛋白编号BC028194
基因名称 VASH2
Gene ID (NCBI) 79805
RRIDAB_10642836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86V25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. This antibody recognizes both the nuclear and cytoplasmic isoforms.(PMID:23615928; 19204325; 31235911; 26177649)

实验方案

Product Specific Protocols
WB protocol for VASH2 antibody 13303-1-APDownload protocol
IHC protocol for VASH2 antibody 13303-1-APDownload protocol
IF protocol for VASH2 antibody 13303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Basic Res Cardiol

Extracellular stiffness induces contractile dysfunction in adult cardiomyocytes via cell-autonomous and microtubule-dependent mechanisms

Authors - Alexia Vite
View Article