验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells, Raji cells, Daudi cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84901-1-RR targets UVRAG in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag30648 Product name: Recombinant human UVRAG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 198-311 aa of BC064837 Sequence: RLHRAQCAIKQTQVTVQKIGKEIEEKLRLTSTSNELKKKSECLQLKILVLQNELERQKKALGREVALLHKQQIALQDKGSAFSAEHLKLQLQKESLNELRKECTAKRELFLKTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | UV radiation resistance associated gene |
| 别名 | UV radiation resistance-associated gene protein, p63, DHTX, 242051C2 |
| 计算分子量 | 699 aa, 78 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC064837 |
| 基因名称 | UVRAG |
| Gene ID (NCBI) | 7405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9P2Y5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UVRAG is a versatile protein that is involved in the regulation of different cellular pathways implicated in membrane trafficking, and is also involved in the regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; Its function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns3P) (PMID:16799551; 18552835; 20643123; 24056303; 28306502).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UVRAG antibody 84901-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
