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USP36 Monoclonal antibody

USP36 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat No : 68165-1-Ig

Print datasheet

Synonyms

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

验证数据展示

  • WB analysis using 68165-1-Ig

    WB analysis using 68165-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68165-1-Ig

    WB analysis using 68165-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68165-1-Ig

    WB analysis of HeLa using 68165-1-Ig

    WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells.

经过测试的应用

Positive WB detected inU2OS cells, K-562 cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68165-1-Ig targets USP36 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman
Immunogen USP36 fusion protein Ag6426 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 36
Synonyms Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36
Calculated Molecular Weight 1123 aa, 123 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC038983
Gene Symbol USP36
Gene ID (NCBI) 57602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P275
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)

实验方案

Product Specific Protocols
WB protocol for USP36 antibody 68165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3

Authors - Gengchen Xie
View Article