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验证数据展示

  • WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP15 antibody included
  • IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP15 antibody included
  • WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP15 Monoclonal antibody, PBS Only

USP15 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67557-1-PBS

产品说明书

CloneNo. 1C9G3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy USP15 Ready-To-Use IHC Kit. USP15 primary antibody included.

产品信息

67557-1-PBS targets USP15 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5712

Product name: Recombinant human USP15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-235 aa of BC020688

Sequence: MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKKPLEQSC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 15
别名 1C9G3, Deubiquitinating enzyme 15, EC:3.4.19.12, KIAA0529, Ubiquitin carboxyl-terminal hydrolase 15
计算分子量 112 kDa
观测分子量 112 kDa
GenBank蛋白编号BC020688
基因名称 USP15
Gene ID (NCBI) 9958
RRIDAB_2882771
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y4E8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.